The Effect of Chronic NaCl Exposure on the Reproduction of Daphnia magna

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Mish, Angela Marie Edwards
Casey, Ellie
Kohler, Paige
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2024-04-04
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"An artificial increase in the salinity of freshwater has many different sources such as mining, irrigation, and de-icing agents on roads. The salinity of freshwater environments is a threat to organisms as many can only tolerate certain ranges when maintaining osmoregulation. Many species are not able to continue biological processes past a certain point of salinity including reproduction and survival. Daphnia magna, a freshwater amphipod has many of the desired traits of an experimental organism: short generation time, easily collected and cultured, and can be sexed, aged, and measured in a short period of time. D. magna are also a favored study species as they are parthenogenetic meaning that they can reproduce asexually producing clones but may also produce males when introduced to environmental stress to facilitate adaptation through the increased genetic diversity provided by sexual reproduction. The objective of the study is to determine if the reproduction rate of consecutive generations is changed based on chronic NaCl exposure. A secondary objective is to investigate if after several generations the offspring of D. magna becomes more resilient than previous generations either in a lower effect concentration or in the production of males. Daphnia magna will be exposed to five sublethal concentrations of NaCl. Each of the 10 replicates will have one adult D. magna, and their time until reproductive maturity, number of broods, brood sizes, and overall life span will be measured. Three generations, including the original mothers, will be observed until death. D. magna are essential components of aquatic ecosystems and impacts on their reproduction may have widescale ecosystem effects."
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